The　electrochemiluminescence　(ECL)　intensity　can　be　regulated　by　ionic　current　passing　through　the　microchannel,　which　broadened　the　regulation　of　the　ECL　sensors.　But　in　the　early　reported　sensors,　the　electrostatic　repulsion　and　steric　hindrance　caused　few　targets　to　approach　the　interface　of　the　microchannel　driven　by　concentration　difference,　which　reduced　the　detection　efficiency　and　prolonged　the　detection　period.　In　this　study,　different　accumulation　strategies,　such　as　a　positive　electric　field　and　different　polarity　electric　fields,　were　designed　to　accumulate　targets　in　the　microchannel.　The　interaction　of　azide　groups　and　hydrogen　sulfide　served　as　a　research　model.　Hydrogen　sulfide　can　react　with　the　negatively　charged　azide　groups　in　the　microchannel　surface　to　produce　positively　charged　amino　groups,　decreasing　the　negative　charge　density　of　the　microchannel　and　thus　altering　the　ionic　current　and　ECL　intensity.　The　accumulation　of　hydrogen　sulfide　at　the　microchannel　tip　can　increase　the　collision　probability　with　azide　groups　to　improve　the　detection　efficiency,　and　the　integration　of　accumulation　and　reaction　can　shorten　the　detection　period　to　28　min.　The　hydrogen　sulfide　concentration　on　the　microchannel　tip　accumulated　by　applying　different　polarity　electric　fields　was　22.3-fold　higher　than　that　accumulated　by　applying　a　positive　electric　field.　The　selected　research　model　broadened　the　application　range　of　a　microchannel-based　ECL　sensor　and　confirmed　the　universality　of　the　microchannel-based　ECL　sensor.　©　2024　American　Chemical　Society.