An　investigation　was　conducted　in　the　vicinity　of　a　sustainable　pig　farm　to　assess　the　presence　of　antibiotics　through　SPE-HPLC/MS/MS,　microbial　communities　via　Illumina　high-throughput　sequencing,　and　antibiotic　resistance　genes　using　SmartChip　technology.　The　study　revealed　that　tetracyclines　were　the　predominant　antibiotics　detected　in　the　soil　and　sediment　surrounding　the　pig　farm,　with　residual　concentrations　ranging　from　33.3　to　1244.2 μg∙kg−1.　The　most　prevalent　phyla　identified　at　various　sampling　sites　were　Firmicutes,　Chloroflexi,　Proteobacteria,　Actinobacteria,　and　Acidobacteria.　A　total　of　188　antibiotic　resistance　genes　(ARGs)　and　9　mobile　genetic　elements　were　found　in　the　sediment,　with　aminoglycoside　(particularly　aadA2-03),　sulfonamide　(specifically　sul2),　and　tetracycline　(particularly　tetX)　resistance　genes　being　the　most　frequently　observed.　The　presence　of　tetracycline　residue　was　observed　to　influence　the　composition　of　the　microbial　community,　whereas　no　significant　association　was　found　between　antibiotics　and　ARGs.　Examination　of　the　correlation　between　ARGs　and　bacteria　at　the　phylum　level　demonstrated　that　Cyanobacteria,　Acidobacteria,　Planctomycetota,　and　Gemmatimonadota　were　the　predominant　phyla　associated　with　ARG　presence　near　an　intensive　pig　farm.　Notably,　Cyanobacteria　may　function　as　a　continual　reservoir　and/or　shelter　for　ARGs,　thereby　potentially　contributing　to　the　dissemination　of　ARGs　in　the　sediment　environment　in　close　proximity　to　a　pig　farm.　This　study　presents　evidence　of　the　ecological　risks　posed　by　antibiotics　in　a　pig　farm-cropland　system,　highlighting　the　connection　between　microbial　community　structure　and　ARGs.　Therefore,　the　issue　of　antibiotic　residues　must　be　factored　into　the　sustainability　of　animal　husbandry　practices.　©　University　of　Tehran　2024.