In　suspected　cases　of　thyroid　toxicity　and　neurotoxicity　in　the　human　body,　the　determination　of　methoxylated　and　hydroxylated　metabolites　of　PBDEs　in　human　serum　samples　is　very　important　for　health　risk　assessment.　In　the　present　paper,　a　sensitive　analytical　method　was　developed　for　the　extraction　and　cleanup　of　PBDEs/MeO-PBDEs　and　OH-PBDEs　in　human　serum.　An　efficient　denaturation　and　extraction　step　was　described,　and　two　methods　for　lipid　removal　were　evaluated.　Recoveries,　based　on　gas　chromatography　coupled　with　mass　spectrometry　in　the　negative　chemical　ionization　mode　(GC-MS-ECNI),　were　between　79.5　and　87.1%　for　the　studied　MeO-PBDEs.　Recoveries　were　between　63.4　and　75.6%　for　the　studied　OH-PBDEs,　which　were　generally　slightly　lower　than　those　of　the　neutral　compounds　but　still　suitable　for　the　analysis　of　these　compounds　in　human　serum.　The　developed　method　was　further　validated　in　five　human　serum　samples　from　electronic　waste　dismantling　workers.　The　results　indicated　that　no　selected　methoxylated　brominated　diphenyl　ethers　metabolites　were　identified,　however,　three　hydroxylated　higher　brominated　diphenyl　ethers　such　as　OH-octaBDEs　and　OH-nonaBDEs　were　identified　in　human　serum.　The　dominant　Sigma　PBDE　and　Sigma　OH-PBDE　concentrations　in　workers＇　serum　ranged　from　5.58　to　228.52　ng　g(-1)　lipid　and　from　44.66　to　896.13　ng　g(-1)　lipid,　respectively.　The　levels　of　hydroxylated　metabolites　of　PBDEs　found　in　the　electronic　waste　dismantling　workers　were　similar　or　even　higher　than　their　precursors.