In　this　study,　a　new　approach　for　the　preparation　of　a　fluorescent　europium(III)　complex-doped　silica　nanoparticles　has　been　developed.　The　synthesis　process　involved　the　following　steps:　(1)　preparing　silica　nanoparticles　by　water-in-oil　microemulsion　method,　(2)　dyeing　the　spherical　silica　particles　by　europium(III):　naphtoyltrifluoroacetone　(NTA):trioctylphosphineoxide　(TOPO),　(3)　adsorbing　polyvinylpyrrolidone　(PVP)　onto　the　core　structure　and　growing　silica　on　PVP　surface.　The　as-prepared　nanoparticles　exhibited　stronger　emission　intensity,　higher　photo-　and　chemical　stability.　Despite　the　fact　that　europium(III)　complex　was　doped　into　the　nanoparticles,　its　fluorescence　properties　such　as　a　wide　Stokes　shift,　a　narrow　emission　peak,　and　long　fluorescence　lifetime,　were　retained.　The　nanoparticles　are　uniform　in　shape　and　size　(50　+/-　A　5　nm　in　diameter).　This　study　could　provide　new　avenue　for　the　fabrication　of　Eu:　NTA:TOPO-based　nanoparticles,　facilitating　their　application　in　bioassay　issues.