Microalgae　with　high　biomass　and　high　lipid　content　are　the　ideal　feedstock　for　biodiesel　production.　To　obtain　such　microalgae,　ultraviolet　(UV)　irradiation　was　applied　to　Chlorella　sp.　to　induce　mutagenesis.　The　growth　characteristics,　total　nitrogen　(TN),　and　biochemical　compositions　of　the　control　and　UV　mutation　strains　were　analyzed.　Compared　to　the　control　strain,　the　biomass　for　the　UV　mutation　strain　was　7.6　%　higher　and　it　presented　a　higher　growth　rate.　The　lipid　content　of　the　UV　mutation　strain　showed　different　levels　of　increase　and　reached　the　maximum　value　of　28.1　%　on　day　15.　Furthermore,　the　lipid　productivity　of　the　UV　mutation　strain　showed　a　desired　increase.　The　nitrogen　consumption　and　Acetyl-CoA　carboxylase　(ACC)　activity　contributed　to　the　lipid　production　by　UV.　All　these　results　indicate　that　UV　mutagenesis　is　an　efficient　method　to　improve　probability　for　using　Chlorella　sp.　as　the　potential　raw　material　for　biodiesel　production.