Polybrominated　diphenyl　ethers　(PBDEs)　are　becoming　a　public　health　concern　because　of　their　potential　toxicity,　from　endocrine　disruption　system　to　neurodevelopmental　impairments.　Nonetheless,　information　on　their　levels　in　human　blood　is　scarce.　In　this　study,　human　serum　samples　collected　in　Shanghai,　China,　were　analyzed　for　the　concentrations　of　PBDEs　and　their　hydroxylated　metabolites　(OH-PBDEs).　Eight　PBDE　congeners　and　six　OH-PBDE　congeners　were　quantified　in　serum　samples　by　gas　chromatography　with　mass　spectrometry　(GC-MS)　and　high-performance　liquid　chromatography　with　tandem　mass　spectrometry　(HPLC-MS/MS).　As　a　result,　total　PBDE　concentration　ranged　from　0.280　to　12.330　ng　g(-1)　on　a　lipid　weight　basis　lw　(median:　1.100　ng　g(-1)　lw)　and　the　total　OH-PBDE　level　ranged　from　0.045　to　0.363　ng　g(-1)　(lw)　(median:　0.187　ng　g(-1)　lw).　Among　them,　BDE-47　and　6-OH-BDE-47　were　the　predominant　PBDEs　and　OH-PBDEs,　respectively.　In　addition,　based　on　the　results　of　the　Bartelett　X-2　test,　BDE-47　significantly　(p　＜　0.05)　correlated　with　BDE-28,　BDE-100,　BDE-85,　and　BDE-154,　whereas　3＇-OH-BDE-7　significantly　(p　＜　0.01)　correlated　with　3-OH-BDE-47,　2-OH-BDE-68,　and　6＇-OH-BDE-99.　Among　all　donors,　no　significant　association　between　age　and　PBDEs　(or　OH-PBDEs)　was　found.　Further　research　on　the　exposure　routes　in　the　environment　and　metabolic　processing　of　PBDEs　in　human　blood　is　necessary.