Controlling　the　structure　and　charge　of　nanofiltration　membranes　is　vital　for　removing　emerging　contaminants　in　both　point-of-use　and　industrial　wastewater　treatment.　However,　the　challenge　is　finding　a　viable　method　to　regulate　interfacial　polymerization　for　high-permeance　polyamide　membranes　with　tailored　selectivity　and　reduced　scaling　potential.　Here　we　propose　a　simple　and　straightforward　approach　to　creating　an　ultra-thin,　highly　charged　polyamide　membrane　through　protonation-regulated　interfacial　polymerization　with　phos-phate　saline　buffer　(PB-IP).　The　resulting　thin　films　exhibit　a　long-term　permeation　performance　with　permeance　＞110　L　m-2　h-1　bar-　1　without　pore　collapsing.　Within　these　membranes,　the　polyamide　network　is　composed　of　loosely　crosslinked　chains　with　high　charge　density,　providing　sub-1　nm　pore　sizes　that　are　effective　for　sepa-rating　small　molecules,　such　as　perfluorooctanoic　acid　(PFOA)　and　different　antibiotics.　The　PB-IP　methodology　allows　for　tailored　membrane　functionality,　particularly　for　reducing　the　rate　of　surface　mineral　deposition.　We　examined　the　inorganic　scaling　propensity　of　these　membranes　by　systematically　quantifying　the　rejection　of　different　salt　species　and　studying　the　change　in　hydraulic　flux　using　the　solution　with　high　gypsum　saturation　index.　Furthermore,　the　fouling　characteristics　of　the　membranes　were　stimulated　with　common　foulants　to　demonstrate　the　antifouling　characteristic.　This　work　provides　a　feasible　strategy　to　adjust　the　structure　-property-performance　relationship　profile　for　polyamide　membranes,　and　to　enable　their　use　in　water　purifi-cation　under　a　more　dynamic　range　of　water　conditions.