Conductive　hydrogels　have　shown　a　great　potential　in　the　field　of　flexible　electronic　devices.　However,　conductive　hydrogels　prepare　by　traditional　methods　are　difficult　to　combine　high　strength　and　toughness,　which　limits　their　application　in　various　fields.　In　this　study,　a　strategy　for　preparing　conductive　hydrogels　with　high　strength　and　toughness　by　using　the　synergistic　effect　of　biomineralization　and　salting-out　was　pioneered.　In　simple　terms,　by　immersing　the　CaCl2　doped　soy　protein　isolate/poly(vinyl　alcohol)/dimethyl　sulfoxide　(SPI/PVA/DMSO)　hydrogel　in　Na2CO3　and　Na3Cit　complex　solution,　the　biomineralization　aroused　by　Ca2+　and　CO32−,　and　the　salting-out　effect　of　both　NaCl　and　Na3Cit　would　enhance　the　mechanical　properties　of　SPI/PVA/DMSO　hydrogel.　Meanwhile,　the　ionic　conductivity　of　the　hydrogel　would　also　increase　due　the　introduction　of　cation　and　anion.　The　mechanical　and　electrical　properties　of　SPI/PVA/DMSO/CaCO3/Na3Cit　hydrogels　were　significantly　enhanced　by　the　synergistic　effect　of　biomineralization　and　salting-out.　The　optimum　tensile　strength,　toughness,　Young＇s　modulus　and　ionic　conductivity　of　the　hydrogel　were　1.4　±　0.08　MPa,　0.51　±　0.04　MPa　and　1.46　±　0.01　S/m,　respectively.　The　SPI/PVA/DMSO/CaCO3/Na3Cit　hydrogel　was　assembled　into　a　strain　sensor.　The　strain　sensor　had　good　sensitivity　(GF　=　3.18,　strain　in　20　%–500　%)　and　could　be　used　to　accurately　detect　various　human　movements.　©　2023