The　present　study　describes　a　novel　method　for　green　synthesis　of　silver　nanoparticles　using　Cnidium　monnieri　(CM-AgNPs).　Cnidium　monnieri　fruit　is　an　excellent　anti　tinea　drug　that　can　be　used　externally　to　treat　superficial　fungal　infections　in　the　human　body.　The　aqueous　ethanolic　extract　of　Cnidium　monnieri　fruit　was　prepared　and　employed　in　the　synthesis　of　stable　silver　nanoparticles　via　biological　reduction　method.　The　synthesis　conditions　of　CM-AgNPs　was　systematically　optimized　using　Box-Behnken　design.　CM-AgNPs　were　well　characterized　by　UV-spectroscopy　and　X-ray　powder　diffraction　(XRD),　and　it　was　confirmed　that　the　synthesized　particles　were　AgNPs.　The　possible　functional　groups　required　for　the　reduction　and　stabilization　of　CM-AgNPs　in　the　extract　were　identified　through　FTIR　spectrum.　The　size　of　CM-AgNPs　structure　was　confirmed　to　be　approximately　44.6　nm　in　polydisperse　spherical　shape　through　scanning　electron　microscopy　(SEM),　transmission　electron　microscopy　(TEM),　and　laser　dynamic　light　scattering　(DLS).　Further,　the　minimum　inhibitory　concentration　90%　(MIC90)　ratios　values　of　Cm-AgNPs　against　Trichophyton　rubrum　(7　d),　T.　mentagrophytes　(7　d)　and　Candida　albicans　(24　h)　were　3.125,　3.125,　and　0.78125　mu　g/mL,　respectively,　determined　by　the　broth　micro　dilution　method.　Finally,　the　result　was　concluded　that　the　synthesized　AgNPs　could　be　further　evaluated　in　large　scale　as　a　potential　human　topical　antifungal　agent.