The　treatment　and　reuse　of　wastewater　are　crucial　for　the　effective　utilization　and　protection　of　global　water　resources.　Polycyclic　aromatic　hydrocarbons　(PAHs),　as　one　of　the　most　common　organic　pollutants　in　industrial　wastewater,　are　difficult　to　remove　due　to　their　relatively　low　solubility　and　bioavailability　in　the　water　environment.　However,　biosurfactants　with　both　hydrophilic　and　hydrophobic　groups　are　effective　in　overcoming　these　difficulties.　Therefore,　a　biosurfactant-producing　strain　Pseudomonas　mosselii　MP-6　was　isolated　in　this　study　to　enhance　the　bioavailability　and　biodegradation　of　PAHs,　especially　high-molecular-weight　PAHs　(HMW-PAHs).　FTIR　and　LC-MS　analysis　showed　that　the　MP-6　surfactant　belongs　to　rhamnolipids,　a　type　of　biopolymer,　which　can　reduce　the　water　surface　tension　from　73.20　mN/m　to　30.61　mN/m　at　a　critical　micelle　concentration　(CMC　=　93.17　mg/L).　The　enhanced　solubilization　and　biodegradation　of　PAHs,　particularly　HMW-PAHs　(when　MP-6　was　introduced),　were　also　demonstrated　in　experiments.　Furthermore,　comprehensive　environmental　stress　tolerance　tests　were　conducted　to　confirm　the　robustness　of　the　MP-6　biosurfactant,　which　signifies　the　potential　adaptability　and　applicability　of　this　biosurfactant　in　diverse　environmental　remediation　scenarios.　The　results　of　this　study,　therefore,　have　significant　implications　for　future　applications　in　the　treatment　of　wastewater　containing　HMW-PAHs,　such　as　coking　wastewater.