Oil　pollution　from　the　petroleum　industry　is　a　growing　problem,　especially　in　terms　of　the　harm　it　causes　to　the　aquatic　environment,　which　puts　humans　and　other　aquatic　life　in　grave　danger.　As　immobilized　carriers　for　the　removal　of　diesel,　thermoplastic　polyurethane　(TPU)　nanofiber　membranes　and　co-blended　nano-hydroxyapatite　modified　thermoplastic　polyurethane　(TPU/nHA)　nanofiber　membranes　are　reasonably　priced,　non-toxic,　and　non-polluting.　The　adhesion　and　colonization　of　the　bacterial　cells　to　these　carriers　were　studied　using　scanning　electron　microscopy　(SEM).　Gas　chromatography　was　used　to　measure　the　bioremediation　effectiveness,　and　the　results　after　3　days　of　remediation　revealed　a　substantial　improvement　in　the　removal　of　hydrocarbons　by　immobilization　compared　to　free　bacteria.　With　an　initial　concentration　of　3　g/L　diesel,　the　TPU/1.5HA-bacteria　system　enhanced　the　removal　of　diesel　the　most　(20.64　±　0.40%),　followed　by　TPU/1HA-bacteria　(17.49　±　0.08%),　TPU/0.5HA-bacteria　(11.97　±　0.40%),　and　TPU-bacteria　(4.69　±　0.13%),　in　that　order.　Within　three　days,　these　scaffolders　had　completely　absorbed　the　spilled　diesel.　There　haven＇t　been　any　studies　done　yet　on　the　removal　of　diesel　using　various　carriers　loaded　with　Bacillus　altitudinis　LS-1.　This　study　demonstrated　that　TPU-based　electrostatic　spinning　films　can　be　used　as　bio-carriers　for　hydrocarbon-degrading　bacteria,　enhancing　the　bioremediation　of　oil-contaminated　water.　©　2023　The　Institution　of　Chemical　Engineers