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author:

Su, W. (Su, W..) [1] | Ni, L. (Ni, L..) [2] | Chen, Y. (Chen, Y..) [3] | Wang, D. (Wang, D..) [4] | Lin, C.-C. (Lin, C.-C..) [5] | Liu, Y. (Liu, Y..) [6] | Liu, Z. (Liu, Z..) [7]

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Scopus

Abstract:

The influence of roasting on tea bitterness remains unclear. With Wuyi Rock tea (WRT) as an example, this study investigated the impact of roasting on WRT's bitterness, utilizing an integrated approach involving sensory evaluation, bitter compound profiling, and cell-based calcium imaging. Sensory analysis revealed that roasting effectively reduced the perceived bitterness of WRT. This reduction was supported by decreases in various bitter compounds, including 19 flavanols, 11 flavonols, 12 phenolic acids, 2 purine alkaloids, and 9 bitter amino acids, which diminished by 16%, 26%, 19%, 2%, and 70%, respectively. Furthermore, we established two heterogeneous bitter receptor expression systems: TAS2R39/Gα15-HEK293T and TAS2R14/Gα15-HEK293T cell lines. These systems quantitatively confirmed the reduction in bitterness, demonstrating 51% and 62% decreases in intracellular calcium mobilization within the transfected cells, respectively. These findings provide compelling evidence for the bitterness-ameliorating effect of roasting, expanding our knowledge of the role of roasting in shaping the flavor of tea. © 2023 Elsevier Ltd

Keyword:

Bitterness Calcium imaging Roasting TAS2Rs Tea

Community:

  • [ 1 ] [Su W.]College of Chemical Engineering, Fuzhou University, Fujian, Fuzhou, 350108, China
  • [ 2 ] [Su W.]Institute of Food Science & Technology, Fuzhou University, Fujian, Fuzhou, 350108, China
  • [ 3 ] [Ni L.]College of Chemical Engineering, Fuzhou University, Fujian, Fuzhou, 350108, China
  • [ 4 ] [Ni L.]Institute of Food Science & Technology, Fuzhou University, Fujian, Fuzhou, 350108, China
  • [ 5 ] [Chen Y.]Institute of Food Science & Technology, Fuzhou University, Fujian, Fuzhou, 350108, China
  • [ 6 ] [Wang D.]College of Chemical Engineering, Fuzhou University, Fujian, Fuzhou, 350108, China
  • [ 7 ] [Wang D.]Institute of Food Science & Technology, Fuzhou University, Fujian, Fuzhou, 350108, China
  • [ 8 ] [Lin C.-C.]Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu, Taiwan, Province of China, China
  • [ 9 ] [Liu Y.]Department of Food Science & Technology, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai, 200240, China
  • [ 10 ] [Liu Z.]Institute of Food Science & Technology, Fuzhou University, Fujian, Fuzhou, 350108, China

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Source :

Food Chemistry

ISSN: 0308-8146

Year: 2024

Volume: 437

8 . 5 0 0

JCR@2023

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count: 2

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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