Electrochemiluminescence　nanoprobes　with　a　core-shell-shell　structure　have　been　designed　and　applied　for　hyaluronidase　detection.　The　nanoprobes　can　precipitate　efficiently　through　target-regulation　wettability　for　collection,　and　enrich　near　to　the　hydrophobic　electrode　surface　through　hydrophobic　interaction　to　enhance　the　performance　of　the　biosensor.　The　electrochemiluminescence　nanoprobes　can　precipitate　efficiently　through　target-regulation　wettability　for　collection,　and　enrich　near　to　the　hydrophobic　electrode　surface　through　hydrophobic　interaction　to　enhance　the　biosensor＇s　performance.