A　novel　three-dimensional　covalent　organic　framework　(3D-COF)　with　content-tunable　and　active　hydroxyl　groups　(OH)　on　the　pore　walls　was　developed　and　adopted　for　the　high-performance　capture　of　okadaic　acid　(OA)　marine　toxins.　Using　pore-surface　engineering,　the　integration　of　linear　building　blocks　(4,4＇-diamino-3,3＇-　biphenyldiol,　BD(OH)2　and　benzidine,　BD)　with　the　3D　structural　building　block　backbone　(4,4＇,4＇,4＇＇＇-methane-tetrayltetrabenzaldehyde,　TFPM)　was　achieved.　By　adjusting　the　ratio　of　BD(OH)2,　functional　multicomponent-COFs　[OH](x)-BD-TFPM　COFs　(X　=　25%)　were　synthesized,　which　offered　ideal　access　to　convert　a　conventional　COF　into　a　functional　platform　with　multiple-mode　interactions　of　hydrophobic　and　hydrophilic　groups　for　OA　capture.　[OH](x)-BD-TFPM　was　characterized　using　SEM,　XRD,　FT-IR,　and　BET.　The　adsorption　features　and　analytical　performance　of　OA　were　screened　and　evaluated.　Optimization　of　dispersive　solid-phase　extraction　using　[OH]25-BD-TFPM　was　accomplished,　and　the　method　was　verified　for　sensitive　quantitative　detection　of　OA　in　clam　and　mussel　samples.　Coupled　with　LC-MS/MS,　the　resultant　[OH]25-BD-TFPM　COF　demonstrated　the　ability　to　analyze　OA,　and　the　limit　of　detection　for　OA　in　shellfish　was　determined　to　be　0.005　mu　g/kg.　A　significant　improvement　in　trace　OA　detection　was　observed　compared　to　previously　reported　SPE　materials　without　adjustable　hydrophilic　interactions.　The　recoveries　of　OA　in　the　fortified　clam　and　mussel　samples　were　in　the　ranges　of　93.9-105.1%　and　96.7-110.2%,　respectively.　This　study　highlights　that　OH-group　surface　engineering　in　channel　walls　is　a　facile　and　powerful　strategy　for　developing　functional　3D-COFs　with　multiple　interactions　for　high-performance　target　capture.