It　is　difficult　to　show　microbial　growth　kinetics　online　when　they　grow　in　complex　matrices.　We　presented　a　novel　strategy　to　address　this　challenge　by　developing　a　high-performance　microbial　growth　analyzer　(HPMGA),　which　employed　a　unique　32-channel　capacitively　coupled　contactless　conductivity　detector　as　a　sensing　element　and　fixed　with　a　CellStatz　software.　It　was　capable　of　online　showing　accurate　and　repeatable　growth　curves　of　well-dispersed　and　bad-dispersed　microbes,　whether　they　grew　in　homogeneous　simple　culture　broth　or　heterogeneous　complex　matrices.　Moreover,　it　could　automatically　report　key　growth　kinetics　parameters.　In　comparison　to　optical　density　(OD),　plate　counting　and　broth　microdilution　(BMD)　methods,　we　demonstrated　its　practicability　in　five　scenarios:　1)　the　illustration　of　the　growth,　growth　rate,　and　acceleration　curves　of　Escherichia　coli　(E.　coli);　2)　the　antimicrobial　susceptibility　testing　(AST)　of　Oxacillin　against　Staphylococcus　aureus　(S.　aureus);　3)　the　determination　of　Ag　nanoparticle　toxicity　on　Providencia　rettgeri　(P.　rettgeri);　4)　the　characterization　of　milk　fermentation;　and　5)　the　enumeration　of　viable　pathogenic　Vibrio　in　shrimp　body.　Results　highlighted　that　the　HPMGA　method　had　the　advantages　of　universality　and　effectivity.　This　technology　would　significantly　facilitate　the　routine　analysis　of　microbial　growth　in　many　fields　(biology,　medicine,　clinic,　life,　food,　environment,　and　ecology),　paving　an　avenue　for　microbiologists　to　achieve　research　goals　that　have　been　inhibited　for　years　due　to　a　lack　of　practical　analytical　methods.　©　2023