Flavonoids　are　widely　used　in　the　treatment　of　various　diseases　due　to　their　antioxidant,　anti-inflammatory,　anticancer　and　antiviral　properties.　Fluorescence　detection　is　rarely　applied　for　the　determination　of　flavonoids　because　of　their　weak　fluorescence.　In　this　work,　a　method　of　fluorescence　enhancement　of　flavonoids　was　firstly　introduced　by　using　sodium　acetate　for　flavonoid　derivatization.　The　study　discovered　that　flavonoids,　with　a　hydroxyl　at　the　C3　position,　had　the　ability　to　emit　strong　fluorescence　after　derivatization.　Five　flavonoids,　kaempferide,　galangin,　isorhamnetin,　kaempferol　and　quercetin,　having　a　special　structure,　were　selected,　derivatized　and　analyzed　by　capillary　electrophoresis　with　laser-induced　fluorescence　detection.　Under　the　optimal　conditions,　the　five　flavonoids　could　be　completely　separated　within　3　minutes.　Good　linear　relationships　were　obtained　for　all　analytes　and　the　limits　of　detection　for　the　five　flavonoids　were　in　the　range　of　1.18-4.67　×　10−7　mol　L−1.　Finally,　the　method　was　applied　to　the　determination　of　flavonoids　in　five　traditional　Chinese　medicines:　aster,　chamomile,　galangal,　tangerine　peel　and　cacumen　biotae.　Flavonoids　were　successfully　found　in　all　these　medicines　by　the　developed　method.　The　recoveries　were　in　the　range　of　84.2-111%.　The　method　developed　in　this　study　was　fast,　sensitive　and　reliable　for　the　determination　of　flavonoids.　©　2023　The　Royal　Society　of　Chemistry.