Salmonella　is　one　of　four　key　global　causes　of　diarrhea,　and　in　humans,　it　is　generally　contracted　through　the　consumption　of　contaminated　food.　It　is　necessary　to　develop　an　accurate,　simple,　and　rapid　method　to　monitor　Salmonella　in　the　early　phase.　Herein,　we　developed　a　sequence-specific　visualization　method　based　on　loop-mediated　isothermal　amplification　(LAMP)　for　the　detection　of　Salmonella　in　milk.　With　restriction　endonuclease　and　nicking　endonuclease,　amplicons　were　produced　into　single-stranded　triggers,　which　further　promoted　the　generation　of　a　G-quadruplex　by　a　DNA　machine.　The　G-quadruplex　DNAzyme　possesses　peroxidase-like　activity　and　catalyzes　the　color　development　of　2,2′-azino-di-(3-ethylbenzthiazoline　sulfonic　acid)　(ABTS)　as　the　readouts.　The　feasibility　for　real　samples　analysis　was　also　confirmed　with　Salmonella　spiked　milk,　and　the　sensitivity　was　800　CFU/mL　when　observed　with　the　naked　eye.　Using　this　method,　the　detection　of　Salmonella　in　milk　can　be　completed　within　1.5　h.　Without　the　involvement　of　any　sophisticated　instrument,　this　specific　colorimetric　method　can　be　a　useful　tool　in　resource-limited　areas.　©　2023　by　the　authors.