Garsubellin　A　is　a　thirty-carbon　meroterpenoid　capable　of　enhancing　the　enzyme　choline　acetyltransferase　whose　decreased　level　is　associated　with　the　symptoms　of　Alzheimer＇s　disease.　Due　to　the　potentially　useful　biological　activity　along　with　the　novel　molecular　architecture,　this　plant　metabolite　has　remained　a　popular　synthetic　target.　Herein　we　report　a　full　account　of　our　synthetic　investigations　that　have　led　to　the　enantioselective　total　synthesis　of　garsubellin　A,　establishing　its　absolute　stereostructure.　The　protecting　group-free,　twelve-step　synthetic　route　has　enabled　the　syntheses　of　the　natural　(−)-garsubellin　A　and　its　unnatural　(+)-antipode.　©　2022　Wiley-VCH　GmbH.