The　effective　separation　of　ammonia　(NH3)　has　important　implication　for　the　conversion　improvement　and　pollution　control　during　the　synthesis　of　NH3.　In　the　present　work,　a　novel　kind　of　non-chloride　deep　eutectic　solvents　(DESs)　were　constructed　from　dihydroxybenzoic　acids　(DHBAs)　and　ethylene　glycol　(EG).　The　DHBA　+　EG　DESs　exhibit　several　weak　acidic　sites　and　hydrogen-bond　sites,　thus　were　proposed　for　NH3　separation.　We　found　that　the　amounts　of　NH3　absorbed　by　DHBA　+　EG　DESs　are　quite　impressive,　particularly　at　reduced　pressures.　The　capacities　can　reach　5.64　and　11.69　mol/kg　at　0.1　and　1　bar　respectively,　when　the　temperature　of　298.2　K　is　considered.　Besides,　after　five　absorption–desorption　cycles,　the　DHBA　+　EG　DESs　can　preserve　the　excellent　NH3　absorption　performance.　The　DHAB　+　EG　DESs　can　also　selectively　absorb　NH3　from　N2　and　H2　in　the　mixed　gas.　The　NH3　absorption　mechanism　of　DHBA　+　EG　DESs　was　further　deeply　illustrated　by　spectroscopic　techniques　and　theoretical　calculations.　©　2023　Elsevier　B.V.