Owing　to　the　high　complexity　of　chip　architecture　and　assay　protocol,　considerable　effort　has　been　directed　toward　the　design　automation　of　continuous-flow　microfluidics　over　the　past　decade.　Existing　methods,　however,　perform　the　corresponding　design　tasks,　including　binding,　scheduling,　placement,　and　routing　separately,　leading　to　serious　gaps　between　different　steps　and　potentially　even　cause　design　failure.　To　overcome　these　drawbacks,　in　this　article,　we　propose　a　one-pass　design　paradigm　for　continuous-flow　microfluidic　lab-on-a-chip　systems,　integrating　all　the　design　steps　into　an　＇organic　whole,＇　which　has　never　been　considered　in　prior　work.　With　the　proposed　paradigm,　all　the　design　tasks　can　be　synchronized　seamlessly　and　performed　in　a　combined　manner,　thereby　eliminating　the　gaps　between　design　steps.　Consequently,　optimized　biochip　architectures　can　be　generated　without　any　design　adjustments　and　modifications.　The　experimental　results　demonstrate　the　effectiveness　of　the　proposed　automation　flows.　©　1982-2012　IEEE.