Photocatalytic　antibacterial　approach　shows　great　potential　in　treating　multidrug-resistant　bacterial　infections.　However,　the　bactericidal　efficiency　heavily　depends　on　the　photocatalytic　activity　of　semiconductor　materials,　which　is　limited　by　the　fast　recombination　of　photogenerated　electron-hole　pairs.　Janus　nano-heterostructures　with　spatial　control　growth　of　TiO2　nanoparticles　(NPs)　at　one　end　of　gold　nanorods　(Au　NRs)　are　designed　via　surface　ligand　regulation　for　photocatalytic　sterilization　and　infected　wound　healing.　The　asymmetric　nanostructure　of　Janus　gold　nanorod-titanium　dioxide　nanoparticles　(Janus　AuNR-TiO2　NPs)　promotes　the　directional　migration　of　charge　carriers　and　is　more　conducive　to　the　spatial　separation　of　electron—hole　pairs.　Moreover,　the　injection　of　hot　electrons　and　enhancement　of　plasmon　near-fields　from　the　surface　plasmon　resonance　(SPR)　effect　further　improve　the　photocatalytic　efficiency　of　Janus　AuNR-TiO2　NPs.　Under　simulated　sunlight　irradiation,　large　amounts　of　reactive　oxygen　species　(ROS)　are　generated　for　photocatalytic　antibacterial　activity.　Enhanced　bactericidal　efficiency　up　to　99.99%　against　methicillin-resistant　Staphylococcus　aureus　(MRSA)　is　achieved　in　vitro.　Furthermore,　Janus　AuNR-TiO2　NPs　exhibit　superior　biocompatibility,　structural　stability,　and　also　remarkably　accelerate　MRSA-infected　wound　healing.　Taking　the　above　all　into　consideration,　Janus　AuNR-TiO2　NPs,　as　an　efficient　antibacterial　photocatalyst,　offers　a　promising　strategy　for　MRSA　infectious　therapy.　[Figure　not　available:　see　fulltext.]　©　2022,　Tsinghua　University　Press.