A　suitable　bearing　capacity　of　foundation　is　critical　for　the　safety　of　civil　structures.　Sometimes　foundation　reinforcement　is　necessary　and　an　effective　and　environmentally　friendly　method　would　be　the　preferred　choice.　In　this　study,　the　potential　application　of　enzyme-induced　carbonate　precipitation　(EICP)　was　investigated　for　reinforcing　a　0.6　m　bedding　layer　on　top　of　clay　to　improve　the　bearing　capacity　of　the　foundation　underneath　an　underground　cable　duct.　Laboratory　experiments　were　conducted　to　determine　the　optimal　operational　parameters　for　the　extraction　of　crude　urease　liquid　and　optimal　grain　size　range　of　sea　sands　to　be　used　to　construct　the　bedding　layer.　Field　tests　were　planned　based　on　orthogonal　experimental　design　to　study　the　factors　that　would　significantly　affect　the　bio-cementation　effect　on　site.　The　dynamic　deformation　modulus,　calcium　carbonate　content　and　long-term　ground　stress　variations　were　used　to　evaluate　the　bio-cementation　effect　and　the　long-term　performance　of　the　EICP-treated　bedding　layer.　The　laboratory　test　results　showed　that　the　optimal　duration　for　the　extraction　of　crude　urease　liquid　is　1　h　and　the　optimal　usage　of　soybean　husk　powder　in　urease　extraction　solution　is　100　g/L.　The　calcium　carbonate　production　rate　decreases　significantly　when　the　concentration　of　cementation　solution　exceeds　0.5　mol/L.　The　results　of　site　trial　showed　that　the　number　of　EICP　treatments　has　the　most　significant　impact　on　the　effectiveness　of　EICP　treatment　and　the　highest　dynamic　deformation　modulus　(Evd)　of　EICP-treated　bedding　layer　reached　50.55　MPa.　The　area　with　better　bio-cementation　effect　was　found　to　take　higher　ground　stress　which　validates　that　the　EICP　treatment　could　improve　the　bearing　capacity　of　foundation　by　reinforcing　the　bedding　layer.　The　field　trial　described　and　the　analysis　introduced　in　this　paper　can　provide　a　practical　basis　for　applying　EICP　technology　to　the　reinforcement　of　bedding　layer　in　poor　ground　conditions.　©　2023　Institute　of　Rock　and　Soil　Mechanics,　Chinese　Academy　of　Sciences