In　reality,　various　sulfonamides　(SAs)　were　alternately　used　in　animal　husbandry　to　avoid　generating　drug　resistance.　Thus,　it　is　crucial　to　develop　simple　and　high-throughput　methods　for　detecting　multiple　or　groups　of　SAs　to　realize　rapid　screening　of　total　SAs　residues　in　foods.　We　herein　developed　a　sensitive　and　efficient　MnO2　nanosheets-mediated　etching　of　gold　nanobipyramids　(AuNBPs),　which　can　generate　more　vivid　color　changes,　and　further　fabricated　a　high-throughput　multicolor　immunosensor　for　the　visual　screening/semi-quantitative　detection　of　6　different　SAs　including　sulfamethazine　(SMZ),　sulfamethoxydiazine　(SMD),　sulfisomidine　(SIM),　sulfamerazine　(SMR),　sulfamonomethoxine　(SMM)　and　sulfaquinoxaline　(SQ)　by　using　AuNBPs　as　signal　and　broad-specificity　anti-SAs　antibody　as　a　bio-receptor.　The　immunosensor　displays　more　vivid　color　changes,　and　has　a　lower　visual　detection　limit　and　excellent　specificity.　It　can　be　applied　to　detect　as　little　as　1.0　ng/mL　of　SMZ,　SMD,　SMR　and　2.0　ng/mL　of　SIM,　SMM,　SQ　by　bare　eye　observation,　and　0.2　ng/mL　of　above　6　SAs　by　UV–visible　spectrophotometry.　The　visual　detection　limit　of　the　immunosensor　is　much　lower　than　the　maximum　residue　limit　of　total　SAs　(100　μg/kg)　in　edible　tissues.　The　immunosensor　was　successfully　applied　to　detect　SMZ,　SMD,　SIM,　SMR,　SMM　and　SQ　in　milk　with　a　recovery　of　84%–106%　and　a　RSD　(n　=　5)　<　8%.　The　success　of　this　study　provided　a　promising　assay　for　the　on-site　rapid　screening　of　SMZ,　SMD,　SIM,　SMR,　SMM　and　SQ　in　food　by　bare　eye　observation.　Importantly,　the　immunosensor　may　be　expended　as　a　general　method　for　the　visual　screening/semi-quantitative　detection　of　the　group　of　other　antibiotics　by　using　the　corresponding　broad-specificity　antibody　as　a　bio-receptor.　©　2023　Elsevier　B.V.