Monascus-fermented　red　mold　rice　(RMR)　has　excellent　physiological　efficacy　on　lipid　metabolism　and　liver　function.　This　study　investigated　the　ameliorative　effects　of　monascin　(MS)　from　RMR　on　alcoholic　liver　injury　(ALI)　in　mice,　and　further　illustrated　its　mechanism　of　action.　Results　indicated　that　dietary　MS　intervention　obviously　ameliorated　lipid　metabolism　and　liver　function　in　mice　with　over-drinking.　In　addition,　MS　intervention　alleviated　alcohol-induced　oxidative　stress　in　the　liver　by　reducing　the　hepatic　activities　of　lactate　dehydrogenase　(LDH)　and　hepatic　levels　of　malondialdehyde　(MDA),　increasing　the　hepatic　activities　of　catalase　(CAT),　superoxide　dismutase　(SOD),　alcohol　dehydrogenase　(ADH)　and　hepatic　levels　of　glutathione　(GSH).　16S　rRNA　amplicon　sequencing　showed　that　excessive　drinking　had　a　significant　effect　on　the　composition　of　the　gut　microbiota　in　mice.　MS　intervention　was　beneficial　to　ameliorate　intestinal　microbiota　dysbiosis　by　elevating　the　proportion　of　Lactobacillus,　Lachnospiraceae_UCG-006,　Coriobacteriales,　etc.,　but　decreasing　the　proportion　of　Staphylococcus,　Muribaculaceae,　Desulfovibrionaceae,　etc.　Additionally,　correlation　analysis　indicated　that　the　key　intestinal　bacterial　taxa　intervened　by　MS　were　closely　related　to　some　biochemical　indicators　of　lipid　metabolism,　liver　function　and　oxidative　stress.　Moreover,　liver　metabolomics　analysis　revealed　that　dietary　MS　supplementation　significantly　regulated　the　levels　of　liver　metabolites　involved　in　taurine　and　hypotaurine　metabolism,　riboflavin　metabolism,　and　purine　metabolism,　etc.　Furthermore,　MS　intervention　regulated　gene　transcription　and　protein　expression　associated　with　lipid　metabolism　and　oxidative　stress　in　the　liver.　In　short,　these　findings　suggest　that　MS　mitigates　alcohol-induced　hepatic　oxidative　damage　through　modulating　the　intestinal　microbiome　and　liver　metabolic　pathway,　and　thus　can　be　served　as　a　functional　component　to　prevent　alcoholic　liver　disease.　©　2022