Worldwide,　arsenic　contamination　has　become　a　matter　of　extreme　importance　owing　to　its　potential　toxic,　carcinogenic　and　mutagenic　impact　on　human　health　and　the　environment.　The　magnetite-loaded　biochar　has　received　increasing　attention　for　the　removal　of　arsenic　(As)　in　contaminated　water　and　soil.　The　present　study　reports　a　facile　synthesis,　characterization　and　adsorption　characteristics　of　a　novel　magnetite　impregnated　nitrogen-doped　hybrid　biochar　(N/Fe3O4@BC)　for　efficient　arsenate,　As(V)　and　arsenite,　As(III)　removal　from　aqueous　environment.　The　as-synthesized　material　(N/Fe3O4@BC)　characterization　via　XRD,　BET,　FTIR,　SEM/EDS　clearly　revealed　magnetite　(Fe3O4)　impregnation　onto　biochar　matrix.　Furthermore,　the　adsorbent　(N/Fe3O4@BC)　selectivity　results　showed　that　such　a　combination　plays　an　important　role　in　targeted　molecule　removal　from　aqueous　environments　and　compensates　for　the　reduced　surface　area.　The　maximum　monolayer　adsorption　(Qmax)　of　developed　adsorbent　(N/Fe3O4@BC)　(18.15　mg/g　and　9.87　mg/g)　was　significantly　higher　than　that　of　pristine　biochar　(BC)　(9.89　&　8.12　mg/g)　and　magnetite　nano-particles　(MNPs)　[7.38　&　8.56　mg/g]　for　both　As(III)　and　As(V),　respectively.　Isotherm　and　kinetic　data　were　well　fitted　by　Langmuir　(R2　=　0.993)　and　Pseudo　first　order　model　(R2　=　0.992)　thereby　indicating　physico-chemical　sorption　as　a　rate-limiting　step.　The　co-anions　(PO43-)　effect　was　more　significant　for　both　As(III)　and　As　(V)　removal　owing　to　similar　outer　electronic　structure.　Mechanistic　insights　(pH　and　FTIR　spectra)　further　demonstrated　the　remarkable　contribution　of　surface　groups　(OH–,　–NH2　and　–COOH),　electrostatic　attraction　(via　H-　bonds),　surface　complexation　and　ion　exchange　followed　by　external　mass　transfer　diffusion　and　As(III)　oxidation　into　As(V)　by　(N/Fe3O4@BC)　reactive　oxygen　species.　Moreover,　successful　desorption　was　achieved　at　varying　rates　up　to　7th　regeneration　cycle　thereby　showing　(N/Fe3O4@BC)　potential　practical　application.　Thus,　this　work　provides　a　novel　insight　for　the　fabrication　of　novel　magnetic　biochar　for　As　removal　from　contaminated　water　in　natural,　engineering　and　environmental　settings.　©　2022　The　Authors