Traditional　separation　materials　based　on　Ti3C2Tx　modification　are　susceptible　to　contamination　by　oily　substances　and　have　single　performance,　which　limits　their　applications　in　treatment　of　actual　oily　wastewater.　Here,　to　improve　the　hydrophilicity　and　stability　of　Ti3C2Tx,　EHL-functionalized　Ti3C2Tx　(EHL-Ti3C2Tx)　was　prepared　by　enzymatic　hydrolysis　lignin　(EHL)　forming　secondary　bonds　with　the　terminal　oxygen-containing　groups　on　the　surface　of　Ti3C2Tx.　EHL-Ti3C2Tx　was　further　coated　on　polyurethane　(PU)　sponge　to　construct　rough　structures,　enabling　the　modified　EHL-Ti3C2Tx　@PU　sponge　superhydrophilic/underwater　superoleophobic　wettability　to　achieve　separation　efficiency　above　99.5%　for　different　oil/water　mixtures.　Especially,　the　maximum　permeation　flux　of　EHL-Ti3C2Tx　@PU　for　cyclohexane/water　mixture　separation　is　up　to　231,321　L　m−2　h−1.　In　addition,　the　functionalized　interface　constructed　by　EHL-　Ti3C2Tx　on　mixed　cellulose　ester　(MCE)　membrane　exhibits　high　permeability　to　malachite　green　and　methylene　blue　cationic　dyes　with　rejection　rates　of　99.1%　and　98.1%,　respectively.　Therefore,　two　separation　materials　obtained　by　decorating　EHL-Ti3C2Tx　on　PU　and　MCE　substrates　have　extremely　potential　in　treating　complex　wastewater.　©　2022　Elsevier　B.V.