A　dispersible　large-sized　graphene　quanturr　dots　assemblies　(LSGQD-NAs)　in　aqueous　solution　with　strong　electrochemiluminescence　activity　was　fabricated　from　graphene　oxide　by　nitric　acid　etching.　LSGQD-1NAs　were　characterized　by　transmission　electron　microscopy　(TEM),　atomic　force　microscopy　(AFM),　Fourier　transform　infrared　spectroscopy　(FTIR)　and　Raman　spectroscopy　(Raman),　etc.　The　results　demonstrated　that　the　large-sized　graphene　quantum　dots　had　an　average　height　of　20　nm　and　contained　abundant　carboxyl　and　hydroxyl　groups.　Strong　ECL　was　studied　in　detail　with　the　coreaction　of　K2S2O8　and　the　maximum　emission　wavelength　at　685　urn.　It　is　:found　that　LSGQD-NAs　can　be　easily　assembled　and　modified　on　the　surface　of　GC　electrode　through　the　pi-pi-bond　action　of　unoxidized　graphene.　This　study　provides　a　new　method　for　the　research　of　ECL　sensor　based　on　graphene　quantum　dots.