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author:

Li, Linyan (Li, Linyan.) [1] | Fang, Xiaojun (Fang, Xiaojun.) [2] | Le, Jingqing (Le, Jingqing.) [3] | Zheng, Yanhui (Zheng, Yanhui.) [4] | Tan, Xiarong (Tan, Xiarong.) [5] | Jiang, Zhou (Jiang, Zhou.) [6] | Li, Hongxia (Li, Hongxia.) [7] | Xu, Jianguo (Xu, Jianguo.) [8] | Xu, Huo (Xu, Huo.) [9]

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EI

Abstract:

MicroRNAs (miRNAs) have been identified as important biomarkers with great significance for diagnosis and treatment of various diseases. However, their unique properties, such as small size, high sequence homology, and low abundance, make quantitative analysis of miRNAs extremely challenging. Herein, we reported a cascade catalytic hairpin assembly (CCHA) for sensitive and selective detection of miRNA with three kinds of hairpin probes (HP1, HP2, and HP3). In the presence of target miRNA, a series of toehold-mediated intermolecular DNA strand displacement and hybridization was activated among HP1, HP2, and HP3 to assembly numbers of DNA nanoobjects. During this period, the fluorescence response was greatly intensified to indicate the presence and expression level of interested target miRNA. We have demonstrated that the proposed method exhibits a high assay sensitivity to detect low concentration target and an excellent sequence specificity to distinguish even a single-nucleotide difference in vitro. Moreover, we also demonstrated that our design enables the intracellular imaging of miRNA in live cancer and normal cells. These results showing the promising potential of our CCHA for powerful biosensing, clinic diagnosis, or prognosis. © 2022 Elsevier B.V.

Keyword:

Diagnosis Diseases DNA RNA

Community:

  • [ 1 ] [Li, Linyan]Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou; 350108, China
  • [ 2 ] [Li, Linyan]College of Materials and Chemical Engineeing, Minjiang University, Fujian, Fuzhou; 350108, China
  • [ 3 ] [Fang, Xiaojun]Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou; 350108, China
  • [ 4 ] [Fang, Xiaojun]College of Materials and Chemical Engineeing, Minjiang University, Fujian, Fuzhou; 350108, China
  • [ 5 ] [Le, Jingqing]Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou; 350108, China
  • [ 6 ] [Zheng, Yanhui]College of Materials and Chemical Engineeing, Minjiang University, Fujian, Fuzhou; 350108, China
  • [ 7 ] [Tan, Xiarong]Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou; 350108, China
  • [ 8 ] [Tan, Xiarong]College of Materials and Chemical Engineeing, Minjiang University, Fujian, Fuzhou; 350108, China
  • [ 9 ] [Jiang, Zhou]Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou; 350108, China
  • [ 10 ] [Li, Hongxia]Department of Oncology, Hefei First People's Hospital, Third Affiliated Hospital of Anhui Medical University, Hefei; 230032, China
  • [ 11 ] [Xu, Jianguo]Engineering Research Center of Bio-process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Anhui, Hefei; 230009, China
  • [ 12 ] [Xu, Huo]Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou; 350108, China
  • [ 13 ] [Xu, Huo]College of Materials and Chemical Engineeing, Minjiang University, Fujian, Fuzhou; 350108, China

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Talanta

ISSN: 0039-9140

Year: 2022

Volume: 250

6 . 1

JCR@2022

5 . 6 0 0

JCR@2023

ESI HC Threshold:74

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 9

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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