Based　on　the　deflection　theory　and　the　characteristics　of　multi-tower　self-anchored　suspension　bridges,　considering　the　influence　of　longitudinal　stiffness　of　main　tower　and　bending　effect　of　stiffening　beam,　the　equilibrium　differential　equation　and　deformation　coordination　equation　of　multi-tower　self-anchored　suspension　bridges　are　established.　By　“replacing　beam”　method,　the　practical　calculation　formula　of　internal　force　and　deformation　of　multi-tower　self-anchored　suspension　bridge　was　deduced,　and　the　corresponding　calculation　program　was　implemented.　The　correctness　of　the　analytical　method　and　calculation　program　was　verified　by　an　example.　The　analytical　method　of　static　analysis　of　multi-tower　self-anchored　suspension　bridges　established　in　this　paper　can　theoretically　explain　the　mechanical　characteristics　of　the　structure,　and　the　calculation　method　has　a　clear　calculation　flow.　The　internal　force　and　deformation　of　the　structure　under　live　load　can　be　approximately　calculated　only　by　inputting　the　main　design　parameters　of　the　structure,　which　is　suitable　for　structural　design　and　parameter　analysis　of　multi-tower　self-anchored　suspension　bridge.　©　2022　by　the　authors.