Vascular　endothelial　growth　factor　(VEGF165)　is　a　signal　protein　that　plays　a　central　role　in　the　regulation　of　angiogenesis　and　can　stimulate　angiogenesis.　The　development　of　highly　sensitive　and　selective　detection　method　for　VEGF165　is　very　important　for　disease　diagnosis　and　follow-up　treatment　monitoring.　In　this　study,　an　electrochemiluminescence　(ECL)　aptasensor　for　VEGF165　has　been　developed　based　on　quench　of　H2O2　toward　Ru(bpy)32+/TPrA　ECL　system　and　RecJf　exonuclease　induced　target　recovery　and　hybridization　chain　reaction　(HCR)　as　amplification　strategy.　The　presence　of　VEGF165　makes　a　large　number　of　glucose　oxidase　(GOD)　fixed　on　the　electrode　surface　through　the　double　signal　amplification　strategies.　The　present　of　GOD　cause　the　production　of　a　large　amount　of　H2O2　near　the　electrode　surface　under　excess　amount　of　glucose,　resulting　in　the　inhibition　of　the　ECL　signal　of　Ru(bpy)32+/Au　nanoparticles　(Ru(bpy)32+/AuNPs)　film　fixed　on　the　electrode　surface.　The　ECL　response　of　the　designed　biosensor　has　a　good　linear　relationship　with　the　logarithm　of　the　concentration　of　VEGF165　in　the　range　of　0.5　pg/mL　to　500　ng/mL　with　a　detection　limit　of　0.2　fg/mL.　The　VEGF165　in　serum　samples　has　been　detected　by　the　proposed　aptasensor　with　satisfactory　results.　©　2022　Elsevier　B.V.