Photodynamic　therapy　(PDT)　has　been　appeared　as　a　promising　clinical　diagnosis　and　treatment　for　malignant　tumor.　However,　its　non-specific　distribution　and　phototoxicity　to　normal　tissues　and　skinspartially　hinders　the　clinical　applications　of　PDT　in　cancer　diagnosis　and　therapy.　Herein,　2,4-dinitrobenzenesulfonyl　chloride,　a　commonly　used　quenching　agent,　is　introduced　to　conjugate　with　5,10,15,20-tetra(4-hydroxyphenyl)porphyrin　for　maximizing　therapeutic　effects　and　minimizing　side　effects　of　the　photosensitizer.　Owing　to　the　conjugated　2,4-dinitrobenzenesulfonyl　chloride,　our　designed　photosensitizer　TDBP　can　be　activated　by　glutathione　(GSH)　and　hydrogen　sulfide　(H2S)　in　solution　and　cancer　cells,　and　then　obtain　recuperative　fluorescence　and　photosensitive　activity.　Moreover,　TDBP　can　achieve　enhanced　phototoxicity　to　GSH　and　H2S　overexpressed　cancer　cells　and　reduced　phototoxicity　to　normal　cells　and　skins.　Overall,　the　dual　responsive　photosensitizer　developed　here　is　a　promising　candidate　for　precisely　therapy　on　GSH　and　H2S　overexpressed　cancer　cells.　©　2022　Elsevier　Ltd