The　current　Covid-19　aggravates　membrane　biofouling　issue　caused　by　bacteria　and　viruses　which　are　widely　present　in　water.　Herein　we　synthesized　a　series　of　polyamide　(PA)-based　membranes　engineered　with　distinct　metal　ions　(Cu2+,　Fe3+)　via　one-step　metal-ligand　ligation　for　forward　osmosis　(FO)　separation.　The　antibacterial　and　desalting　behavior　of　membrane　were　investigated　by　systematically　varying　the　influential　factors　including　the　charge　status,　complexation　ability　and　antibacterial　mechanism　of　metal　ions　as　well　as　testing　conditions.　All　the　newly　synthesized　membranes　exhibit　better　performance　with　markedly　increased　water　permeability　and　selectivity.　Therein　the　Fe3+　-　membrane　increases　water　fluxes　by　93%　(FO　mode)　and　112%　(PRO　mode)　relative　to　the　nascent　PA　membrane　with　0.5　M　NaCl　as　the　draw　solution.　Both　Cu2+　and　Fe3+　on　membrane　surface　dramatically　improve　the　membrane　bactericidal　efficacy　against　Escherichia　coli　via　destroying　the　bacterial　phospholipid　layer.　Remarkably,　the　metal　ions　on　membrane　surface　are　easily　regenerated　after　being　consumed　by　bacteria　by　simply　immersing　the　membranes　into　the　corresponding　nitrate　solutions.　The　separation　performance　and　antibacterial　properties　of　the　regenerated　membranes　are　comparable　to　those　of　the　fresh　membranes.　When　using　brine　from　reverse　osmosis　process　as　the　draw　solution,　the　Fe3+　membrane