Arylsulfatase　is　useful　in　industrial　agar　processing　by　removing　sulfate　groups.　A　full-length　arylsulfatase　gene,　designated　ArySMA1,　was　obtained　from　marine　bacteria　Serratia　sp.　SM1.　The　ArySMA1　gene　encoded　a　novel　serine-type　arylsulfatase　and　the　enzymatic　properties　were　characterized.　The　enzyme　presented　notable　capacity　of　removing　sulfate　groups　from　natural　algae　substrates.　Kinetic　study　suggested　that　the　microscopic　thermal　inactivation　rate　of　ArySMA1　in　free　form　was　smaller　than　that　of　the　enzyme-substrate　complex.　The　presence　of　substrate　could　unexpectedly　accelerate　ArySMA1　to　deactivate　at　high　temperature.　Such　phenomenon　was　opposite　to　many　findings　that　substrate　could　stabilize　enzymes　against　heat.　Molecular　dynamics　simulation　and　ANS　fluorescent　assay　indicated　the　substrate　led　the　hydrophobic　regions　of　the　active　site　more　flexible　and　the　sulfate　group　of　the　substrate　could　retard　the　processivity　of　ArySMA1　catalysis.　This　study　provides　guidance　for　agar　desulfation　and　down-stream　processing　industry.　©　2021　Elsevier　Inc.