Objective:　To　propose　a　novel　signal　enhancement　strategy　based　on　the　synergy　between　beta-CD-CuNCs　and　multi-walled　carbon　nanotubes　(MWCNTs)　for　the　detection　of　DNA　oxidative　damage　biomarker　8-Hydroxy-2＇-deoxyguanosine　(8-OHdG).　Methods:　The　sensor　was　constructed　with　the　beta-CD-CuNCs-MWCNTs-nafion　film　and　successfully　used　for　the　quantitative　detection　of　8-OhdG　in　the　presence　of　biomolecules　such　as　ascorbic　acid　(AA)　and　uric　acid　(UA).　To　investigate　the　surface　morphology　of　the　modified　electrode,　Transmission　Electron　Microscopy　(TEM),　Cyclic　Voltammetry　(CV)　and　Electrochemical　Impedance　Spectroscopy　(EIS)　were　performed　on　bare　and　modified　electrodes.　Results:　According　to　Differential　Pulse　Voltammetry　(DPV)　results,　there　was　a　linear　relationship　between　peak　current　and　concentration　of　8-OhdG,　ranging　from　1.0x10(-7)　to　1.0x10(-6)　mol/L　(R-2=0.9926)　and　1.0x10(-6)　to　2.0x10(-5)　mol/L　(R-2=0.9933).　The　detection　limit　(S/N=3)　was　33　nmol/L.　Conclusions:　The　proposed　sensor　had　been　successfully　applied　to　the　determination　of　8-OHdG　in　human　urine　samples　with　high　recovery　rates.