Translated Title

Multicolor Immunodiagnostic for Semiquantitative Visual Detection Syndrome Coronavirus 2 Specific Antibody:A Prospect Strategy for On-site COVID-19 Therapeutic Process Monitoring

Translated Abstract

Rapid　detection　of　body　fluid　severe　acute　respiratory　syndrome　coronavirus　2(SARS-CoV-2)　antibody　is　an　effective　strategy　for　infection　therapeutic　effect　of　coronavirus　disease(COVID-19).　Most　detec?tion　methods　require　relatively　large　equipment,which　limited　their　on-site　application.　Lateral　flow　immuno?assay(LFIA)　can　be　used　to　qualitative　antibody　detection　based　on　the　aggregation　of　gold　nanoparticles　(Au　NPs),which　exhibits　just　one-color　change　and　cannot　realize　rapid　quantitative　detection　without　the　help　of　additional　equipment.　In　this　study,a　high-resolution　multicolor　colorimetric　strategy　was　developed　and　applied　to　assessing　antibody　concentration　at　a　glance　based　on　etching　of　gold　nanorods(Au　NRs).　Firstly,SARS-CoV-2　recombinant　antigen　was　immobilized　on　the　surface　of　the　96-wells.　Then,horseradish　peroxidase(HRP)-labeled　second　antibody　combined　with　antibody　to　form　an　antigen-antibody-secondary　antibody　complex　on　the　well　surface,which　has　direct　relationship　with　antibody　concentration　in　the　sample　and　can　be　used　to　oxidize　3,3′,5,5′-tetramethylbenzidine(TMB)to　form　TMB2+at　the　presence　of　HRP.　The　generation　of　TMB2+　efficiently　etch　Au　NRs　to　produce　multicolor　solution.　The　etching　result　in　vivid　color　changes　in　the　system　has　a　relationship　with　the　amount　of　SARS-CoV-2　IgM　antibody.　Under　the　optimal　conditions,the　proposed　strategy　exhibited　a　linear　response　in　the　5.00―200　IU　concentration　range,and　a　detection　limit　of　1.29　IU　for　SARS-CoV-2　IgM　antibody,with　high　sensitivity　and　specificity.　This　assay　is　prospective　for　the　on-site　semi-quantitative　visual　detection　of　SARS-CoV-2　IgM　antibody　concentration　in　the　COVID-19　therapeutic　process.

Translated Keyword

Antibody
Multicolor colorime-tric strategy
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
Therapeutic process monitoring

Classification

O657

Type

Project Name

Project No.

Access Number

WF:perioarticalgdxxhxxb202111021

Language

Chinese

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