Based　on　the　different　electrochemical　oxidation　potentials　of　metal　ions,　a　new　method　for　the　simultaneous　detection　of　carcinoembryonic　antigen　(CEA)　and　platelet-derived　growth　factor-BB　(PDGF-BB)　was　constructed.　The　amino-rich　polyethyleneimine　gold　nanoparticles　(PEI-Au)　is　used　as　a　signal　amplification　platform　carrier　to　load　aptamers　and　metal　ions.　On　the　other　hand,　the　good　conductivity　of　PEI-Au　can　enhance　the　electrode　conductivity　and　accelerate　electron　transfer.　It　showed　a　good　linear　relationship　in　the　range　of　0.1–26.0　ng　/　mL　and　0.5–20.0　ng　/　mL　while　the　detection　limits　were　33.0　pg　/　mL　and　166.0　pg　/　mL,　respectively.　In　addition,　the　prepared　electrochemical　sensor　has　high　selectivity　and　stability.　Clinical　serum　analysis　results　show　that　the　proposed　sensor　has　potential　application　value　in　the　clinical　detection　of　CEA　and　PDGF-BB.　©　2021　Elsevier　Ltd