Pressure-based　immunoassays　have　been　studied　for　point-of-care　testing　for　which　increasing　the　sensitivity　is　still　a　challenge.　In　this　study,　we　described　an　enhanced　pressure-based　immunoassay　with　a　versatile　electronic　sensor　for　the　sensitive　biological　analysis.　The　versatile　electronic　sensor　had　multifunctional　sensing　capabilities　with　temperature　and　pressure　recording.　Magnetic　bead-modified　capture　antibody　and　platinum　nanoparticle-labeled　detection　antibody　were　used　as　the　biorecognition　element　of　the　target　carcinoembryonic　antigen　(CEA)　(as　a　model　analyte)　and　would　form　a　sandwich-type　immune　complex　with　CEA.　After　simple　magnetic　separation,　this　complex　was　transferred　into　the　detection　chamber,　which　contained　both　hydrogen　peroxide　(H2O2)　and　3,3′,5,5′-tetramethylbenzidine　(TMB).　With　the　catalytic　ability　of　PtNPs,　the　＇H2O2-TMB-PtNPs＇system　was　catalyzed　to　generate　a　large　amount　of　oxygen　(O2)　and　photothermal　agent　of　oxidizer　TMB　(ox-TMB).　Meanwhile,　in　a　sealed　chamber,　further　irradiation　with　an　808　nm　near-infrared　laser　led　to　a　triple-step　signal　amplification　strategy　of　pressure　increase,　temperature　increase,　and　gas　thermal　expansion　to　receive　a　strong　electrical　signal　through　the　electronic　sensor　in　real　time.　Thus,　the　amplified　electrical　signal　from　the　electronic　sensor　could　reveal　the　target　concentration.　In　addition,　we　also　verified　that　the　synergistic　system　with　two　physical　quantities　had　a　lower　limit　of　detection　and　a　wider　detection　range　compared　to　the　detection　system　with　a　single　physical　quantity.　In　general,　this　immunoassay　not　only　helped　in　exploring　an　effective　signal　amplification　pathway　but　also　offered　an　opportunity　for　the　development　of　versatile　electronic　sensors　in　point-of-care　settings.　©　2021　American　Chemical　Society.