Ferroptosis　is　a　novel　iron-dependent　cell　death　pathway　mainly　caused　by　an　abnormal　redox　state　and　associated　with　various　diseases　including　cancer.　Recently,　much　attention　has　been　paid　to　natural　compounds　that　are　involved　in　its　activation　and　inhibition.　This　is　the　first　ever　study　to　demonstrate　the　role　of　juglone　isolated　from　Carya　cathayensis　green　peel　in　inducing　autophagy　and　inhibiting　endometrial　cancer　(EC)　cell　migration.　Subsequently,　Fe2+　accumulation,　lipid　peroxidation,　GSH　depletion,　the　upregulation　of　HMOX1,　and　heme　degradation　to　Fe2+　were　reported.　Juglone　was　involved　in　inducing　autophagy　and　inhibiting　cell　migration　and　endoplasmic　reticulum　stress,　which　are　the　new　hallmarks　of　cancer　treatment.　Collectively,　our　data　indicate　that　juglone　as　a　functional　food　ingredient　induces　the　programmed　cell　death　of　EC　cells　by　activating　oxidative　stress　and　suggest　a　novel　therapeutic　approach　for　the　treatment　and　prevention　of　EC.　This　journal　is　©　The　Royal　Society　of　Chemistry.