In　recent　years,　the　problem　of　progressive　collapse　of　building　structures　has　aroused　widespread　concern　at　home　and　abroad.　As　one　of　the　most　commonly　used　structure　systems　in　engineering　practice,　the　reinforced　concrete　(RC)　frame　structures　have　been　widely　involved　in　many　existing　progressive　collapse　studies.　However,　most　of　the　studies　were　mainly　based　on　deterministic　and　static　analyses　of　column　removal　scenarios.　In　this　study,　the　finite　element　(FE)　model　of　a　typical　RC　frame　beam-column　substructure　is　built　based　on　OpenSees.　Both　static　and　dynamic　progressive　collapse　analyses　of　the　beam-column　substructure　are　performed　and　validated　against　the　experimental　results.　Furthermore,　several　structural　models　were　generated　for　the　uncertainty　analyses　by　the　Latin　hypercube　sampling　method.　The　influences　of　various　uncertainty　factors　are　discussed,　including　the　sectional　geometries,　material　properties　and　so　on.　The　results　indicate　that　the　catenary　action　plays　a　crucial　role　in　RC　frame　beam-column　substructures　against　progressive　collapse.　However,　the　use　of　0.2　rad　chord　rotation　as　the　failure　index　in　the　dynamic　progressive　collapse　analyses　is　to　a　certain　extent　conservative.　Moreover,　according　to　the　sensitivity　analyses,　the　yield　strength　and　ultimate　strength　of　the　longitudinal　reinforcement　are　the　major　factors　that　affect　the　progressive　collapse　resistance　of　RC　frame　beam-column　substructures.　Copyright　©2021　Engineering　Mechanics.　All　rights　reserved.