Using　recycled　aggregate　in　concrete　is　effective　in　recycling　construction　and　demolition　waste.　It　is　of　critical　significance　to　understand　the　fatigue　properties　of　recycled　aggregate　concrete　(RAC)　to　implement　it　safely　in　structures　subjected　to　repeated　or　fatigue　load.　In　this　study,　a　series　of　fatigue　tests　was　performed　to　investigate　the　compressive　fatigue　behavior　of　RAC.　The　performance　of　interfacial　transition　zones　(ITZs)　was　analyzed　by　nanoindentation.　Moreover,　the　influence　of　ITZs　on　the　fatigue　life　of　RAC　was　discussed.　The　results　showed　that　the　fatigue　life　of　RAC　obeyed　the　Weibull　distribution,　and　the　S-N-p　equation　could　be　obtained　based　on　the　fitting　of　Weibull　parameters.　In　the　high　cycle　fatigue　zone　(N≥104),　the　fatigue　life　of　RAC　was　lower　than　that　of　natural　aggregate　concrete　(NAC)　under　the　same　stress　level.　The　fatigue　deformation　of　RAC　presented　a　three-stage　deformation　regularity,　and　the　maximum　deformation　at　the　point　of　fatigue　failure　closely　matched　the　monotonic　stress-strain　envelope.　The　multiple　ITZs　matched　the　weak　areas　of　RAC,　and　the　negative　effect　of　ITZs　on　the　fatigue　life　of　RAC　in　the　high　cycle　fatigue　zone　was　found　to　be　greater　than　that　of　NAC.　©　2021　by　the　authors.　Licensee　MDPI,　Basel,　Switzerland.