The　sustainable　development　of　the　food　industry　is　hindered　by　the　threat　of　drug　resistance,　which　is　caused　by　the　overuse　of　antibiotics.　Fortunately,　marine　fish-derived　antimicrobial　peptides　with　broad-spectrum　activity　are　considered　to　be　the　best　new　antibiotic　substitutes　in　the　future.　Therefore,　discovering　more　antimicrobial　peptides　from　marine　fish　and　exploring　its　application　in　the　field　of　food　safety　have　become　a　hot　research　topic.　This　paper　reviews　recent　progress　in　understanding　the　structure,　biological　activity　and　mechanism　of　action　of　antimicrobial　peptides　derived　from　marine　fish,　and　it　discusses　future　prospects　for　the　application　of　the　antimicrobial　peptides　in　food　safety.　This　paper　is　expected　to　provide　evidence　rationale　for　the　research　and　utilization　of　antimicrobial　peptides　derived　from　marine　fish.　©　2021,　China　Food　Publishing　Company.　All　right　reserved.