Continuous-flow　microfluidic　biochips　(CFMBs),　also　known　as　lab-on-a-chip　platforms,　have　attracted　considerable　attentions　in　the　past　two　decades　and　have　been　widely　used　in　various　laboratory　procedures　in　biomedicine　and　biochemistry　such　as　point-of-care　diagnosis　and　protein　crystallization.　Besides　a　completely　automatic　execution　procedure,　these　micrometerscale　devices　offer　several　advantages　over　the　conventional　fluidic　platforms,　e.g.,　low　sample/reagent　consumption,　low　manufacturing　cost,　and　high　execution　efficiency.　However,　similar　to　the　early　development　of　integrated　circuits,　as　the　feature　size　of　biochips　keeps　shrinking,　tens　of　thousands　microvalves　can　now　be　integrated　into　a　single　chip.　Consequently,　traditional　manual-based　chip　design　is　no　longer　suitable　and　Computer-aided　Design　(CAD)　tools　have　to　be　introduced　to　deal　with　the　large-scale　integration　of　such　chips.　This　paper　presents　a　comprehensive　review　of　recent　advances　in　the　design　automation　of　CFMBs,　including　CAD　techniques　for　architecture　synthesis,　volume　assignment　and　sample　preparation,　testing,　fault-tolerant　design,　and　washing.　These　tools　have　great　significance　in　advancing　the　automation　level　of　biochip　design　and　further　promote　their　wide　adoption.　Finally,　future　trends　and　potential　research　topics　are　discussed　in　detail　to　further　improve　the　performance　of　CFMBs.　©　2021