Tumor　necrosis　factor　(TNF)　plays　an　important　role　in　an　inflammatory　cytokine　storm.　Over-secretion　of　TNF　by　the　host　in　response　to　infection　aggravates　the　disease.　TNF　expression　level　is　positively　correlated　with　the　mortality　caused　by　some　bacterial　infections.　Therefore,　using　TNF　antibody　may　alleviate　the　inflammation　to　resist　bacterial　infections.　The　function　of　fish　TNF-b　antibody　in　bacterial　infection　is　still　unclear.　In　this　study,　infection　models　of　Vibrio　vulnificus　FJ03-X2　strain　with　high　pathogenicity　and　strong　virulence　were　established　in　zebrafish　(Danio　rerio)　fibroblast　cell　line　(ZF4　cells)　and　zebrafish.　Zebrafish　tnfb　(Zetnf-b)　gene　was　cloned　and　expressed　by　Escherichia　coli　BL21　(DE3),　and　Zetnf-b　polyclonal　antibody　was　prepared.　Pre-injection　of　Zetnf-b　polyclonal　antibody　and　AG-126　before　infecting　with　V.　vulnificus　could　increase　the　survival　rate　of　zebrafish　by　36.6　and　46.7%,　respectively.　Pre-injection　of　Zetnf-b　polyclonal　antibody　could　effectively　decrease　the　mortality　of　zebrafish　infected　by　V.　vulnificus.　Thus,　TNF　polyclonal　antibody　therapy　could　be　considered　as　an　effective　strategy　to　control　V.　vulnificus　in　fish.