Although　tumor-derived　exosomes　play　an　important　role　in　the　process　of　metastasis,　differences　in　exosomes　secreted　by　the　same　cells　at　different　stages　or　conditions　have　not　been　noticed　by　most　of　the　relevant　researchers.　Here　we　developed　a　lung　cancer　model　in　nude　mice,　and　the　phenotype　and　inclusions　of　exosomes　secreted　by　early　and　advanced　tumors　were　analysed.　The　size　distribution　and　surface　topography　of　these　two　exosomes　were　not　significantly　different,　but　the　expression　of　CD63　in　early　tumor　exosome　(E-exosome)　was　significantly　lower　than　that　in　advanced　tumor　exosome　(A-exosome).　alpha-SMA　expression　on　HELF　cells　treated　with　A-exosome　was　significantly　higher　than　that　treated　with　E-exosome.　The　ability　of　A-exosome　to　promote　the　migration　of　A549　cells　was　better　than　E-exosome.　Furthermore,　small　RNA　sequence　showed　that　only　3　of　the　171　detected-small　RNAs　were　expressed　simultaneously　in　both　exosomes.　These　findings　proved　that　there　are　significant　differences　in　inclusions　and　functions　between　the　early　and　late　exosomes　of　the　same　tumor.　The　study　highlights　the　importance　of　exosomes　in　cancer　metastasis,　and　might　suggest　exosomes　can　be　used　as　biomarkers　and　therapeutic　targets　for　cancer　metastasis.