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author:

Wu, Huihuang (Wu, Huihuang.) [1] | Gao, Yueming (Gao, Yueming.) [2] (Scholars:高跃明) | Yang, Jiejie (Yang, Jiejie.) [3] | Vai, Mangi (Vai, Mangi.) [4] | Du, Min (Du, Min.) [5] | Pun, Siohang (Pun, Siohang.) [6]

Indexed by:

EI SCIE

Abstract:

Fluorescence immunochromatographic assay (FICA) is a quantitative detection technique widely used in clinical diagnosis, environmental monitoring, and food safety. To deal with the limited applications caused by insufficient detection range, this paper proposes a photoelectric adjustment system suitable for FICA strip readers to expand its detection range. The photoelectric adjustment system is proposed based on the relationship between the excitation light intensity and the fluorescence intensity, which provides the optimal excitation light intensity and a stable baseline amplitude for the FICA strip reader. To verify the proposed system, it was applied to the strip reader we had previously developed. The results show that the linear detection range of the FICA strip reader was extended from 1.95-256 mu g/mL to 1-1024 mu g/mL after using the proposed method. At the same time, the accuracy of the FICA strip reader does not deteriorate and is in good comparisons with the conventional ESEQuant Lateral Flow Reader (with R-2 > 0.9987). Therefore, the proposed photoelectric adjustment method and system can improve the detection range of the strip reader or other similar devices.

Keyword:

detection range Fluorescence Fluorescence immunochromatographic assay Licenses light intensity Optical imaging Optical reflection photoelectric adjustment Safety Strips Voltage control

Community:

  • [ 1 ] [Wu, Huihuang]Fuzhou Univ, Coll Phys & Informat Engn, Fuzhou 350108, Peoples R China
  • [ 2 ] [Gao, Yueming]Fuzhou Univ, Coll Phys & Informat Engn, Fuzhou 350108, Peoples R China
  • [ 3 ] [Yang, Jiejie]Fuzhou Univ, Coll Phys & Informat Engn, Fuzhou 350108, Peoples R China
  • [ 4 ] [Du, Min]Fuzhou Univ, Coll Phys & Informat Engn, Fuzhou 350108, Peoples R China
  • [ 5 ] [Wu, Huihuang]Key Lab Med Instrumentat & Pharmaceut Technol Fuj, Fuzhou 350108, Peoples R China
  • [ 6 ] [Gao, Yueming]Key Lab Med Instrumentat & Pharmaceut Technol Fuj, Fuzhou 350108, Peoples R China
  • [ 7 ] [Yang, Jiejie]Key Lab Med Instrumentat & Pharmaceut Technol Fuj, Fuzhou 350108, Peoples R China
  • [ 8 ] [Vai, Mangi]Key Lab Med Instrumentat & Pharmaceut Technol Fuj, Fuzhou 350108, Peoples R China
  • [ 9 ] [Du, Min]Key Lab Ecoind Green Technol Fujian Prov, Nanping 354300, Peoples R China
  • [ 10 ] [Vai, Mangi]Univ Macau, State Key Lab Analog & Mixed Signal VLSI, Macau 999078, Peoples R China
  • [ 11 ] [Pun, Siohang]Univ Macau, State Key Lab Analog & Mixed Signal VLSI, Macau 999078, Peoples R China
  • [ 12 ] [Vai, Mangi]Univ Macau, Fac Sci & Technol, Dept Elect & Comp Engn, Macau 999078, Peoples R China

Reprint 's Address:

  • 高跃明

    [Gao, Yueming]Fuzhou Univ, Coll Phys & Informat Engn, Fuzhou 350108, Peoples R China;;[Gao, Yueming]Key Lab Med Instrumentat & Pharmaceut Technol Fuj, Fuzhou 350108, Peoples R China

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Source :

IEEE PHOTONICS JOURNAL

ISSN: 1943-0655

Year: 2021

Issue: 3

Volume: 13

2 . 2 5

JCR@2021

2 . 1 0 0

JCR@2023

ESI Discipline: PHYSICS;

ESI HC Threshold:87

JCR Journal Grade:3

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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