Asparanin　A　(AA),　a　natural　compound　present　in　vegetables　and　medicinal　herbs　like　Asparagus　officinalis　L.,　has　been　investigated　extensively　for　its　pharmacological　attributes.　So　far,　the　effect　of　AA　on　endometrial　cancer　(EC)　cell　migration　and　invasion　has　not　been　explored.　Herein,　we　elucidated　the　anti-metastasis　mechanism　of　AA　on　Ishikawa　cells　based　on　miRNA-seq　and　mRNA-seq　integrated　analyses.　AA　treatment　led　to　altered　miRNAs　expression　in　Ishikawa　cells　and　inhibited　the　cell　wound　healing,　cell　migration　and　invasion.　Gene　Ontology　and　KEGG　enrichment　analyses　showed　that　the　target　genes　of　different　expression　miRNAs　were　significantly　enriched　in　Ras,　Rap1　and　MAPK　signaling　pathways.　Further　verification　of　these　changes　via　qRTPCR　and　Western　blot　assays　in　vitro　and　in　vivo　demonstrated　that　AA　could　suppress　human　EC　cell　migration　and　invasion　through　Ras/ERK/MAPK　pathway.　Furthermore,　top　two　miRNAs　(miR-6236-p5　and　miR12136_R+8)　and　top　three　target　genes　(KITLG,　PDGFD,　and　NRAS)　were　identified　as　functional　hub　miRNAs　and　genes　through　miRNA-target　gene　network　analysis.　Our　data　presented　a　holistic　approach　to　comprehend　the　anti-metastatic　role　of　AA　in　EC　after　in　vitro　and　in　vivo　analyses.