Glucomannans　(GMs)　fromabundant　natural　resources　have　excellent　processing　properties　and　plentiful　bioactivities.　In　current　study,　functional　properties　of　GMs　with　different　structural　characteristics,　including　KGM　fromkonjac,　DOP　from　dendrobium,　AGP(40),　ASP-4N,　ASP-6N,　&　ASP-8N　from　aloe　were　determined.　Results　suggested　that　molecular　weights　(M-w)　of　GMs　were　positively　correlated　with　their　water　absorption　capacity,　fat　absorption　capacity,　and　viscosity,　while　ratio　of　mannose/glucose　showed　negative　effect.　Higher　degree　of　acetylation　(DA)　mainly　corresponded　to　higher　values　of　solubility　and　zeta-potential.　Then,　effects　of　the　six　GMs　on　general　health　status,　serumbiochemicals,　and　intestinal　SCFAs　production　in　mice　were　evaluated　in　vivo.　Analysis　of　general　health　status　and　levels　of　serum　biochemicals　revealed　thatmicewith　consecutive　supplementation　of　GMs　for　14　days　performed　normally　comparedwith　those　in　control　group.　Interestingly,　the　productions　of　SCFAs　(mainly　acetate　and　butyrate)　in　the　cecal　and　colonic　contents　were　significantly　promoted.　Generally,　higher　concentrations　of　SCFAs　were　produced　when　mice　were　treated　with　GMs　having　higher　M-w,　ratio　of　glucose,　and　DA.　The　current　investigation　suggested　that　both　functional　and　intestinal　fermentation　property　of　GMs　were　jointly　determined　by　themonosaccharide　composition,　molecular　weight,　and　degree　of　acetylation.　(C)　2020　Elsevier　B.V.　All　rights　reserved.