A　simple　and　feasible　pH　meter-based　immunoassay　is　reported　for　detection　of　C-reactive　protein　(CRP)　using　glucose　oxidase　(GOD)-conjugated　dendrimer　loaded　with　platinum　nanozyme.　Initially,　platinum　nanozymes　were　loaded　into　the　dendrimers　through　an　in　situ　synthetic　method.　Then,　GOD　and　monoclonal　anti-CRP　antibody　with　a　high　molar　ratio　were　covalently　conjugated　onto　carboxylated　dendrimers　via　typical　carbodiimide　coupling.　The　immunoreaction　was　carried　out　with　a　competitive　mode　in　a　CRP-coated　microplate.　Along　with　formation　of　immunocomplex,　the　added　glucose　was　oxidized　into　gluconic　acid　and　hydrogen　peroxide　by　GOD,　and　the　latter　was　further　decomposed　by　platinum　nanozyme,　thus　accelerating　chemical　reaction　in　the　positive　direction.　The　produced　gluconic　acid　changed　the　pH　of　detection　solution,　which　was　determined　using　a　handheld　pH　meter.　Under　optimum　conditions,　the　pH　meter-based　immunoassay　gave　a　good　signal　toward　target　CRP　from　0.01　to　100　ng　mL(-1).　The　limit　of　detection　was　5.9　pg　mL(-1).　An　intermediate　precision　＜=　11.2%　was　acquired　with　batch-to-batch　identification.　No　nonspecific　adsorption　was　observed　during　a　series　of　procedures　to　detect　target　CRP,　and　the　cross-reaction　against　other　biomarkers　was　very　low.　Importantly,　our　system　gave　well-matched　results　for　analysis　of　human　serum　samples　relative　to　a　referenced　ELISA　kit.Graphical　abstract