The　application　of　monolithic　materials　as　carriers　for　enzymes　has　rapidly　expanded　to　the　realization　of　flow-through　analysis　and　bioconversion　processes.　This　expansion　is　partly　attributed　to　the　absence　from　diffusion　limitation　in　many　monoliths-based　enzyme　reactors.　Particularly,　the　relatively　ease　of　introducing　functional　groups　renders　polymer　monoliths　attractive　as　enzyme　carriers.　After　summarizing　the　motivation　to　develop　enzymatic　reactors　using　polymer　monoliths,　this　review　reports　the　most　recent　applications　of　such　reactors.　Besides,　the　present　review　focuses　on　the　crucial　characteristics　of　polymer　monoliths　affecting　the　immobilization　of　enzymes　and　the　processing　parameters　dictating　the　performance　of　the　resulting　enzymatic　reactors.　This　review　is　intended　to　provide　a　guideline　for　designing　and　applying　flow-through　enzymatic　reactors　using　polymer　monoliths.