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author:

李锋 (李锋.) [1] | 邱小明 (邱小明.) [2] | 石贤爱 (石贤爱.) [3] | 郭养浩 (郭养浩.) [4]

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Abstract:

建立了一种硫酸新霉素的HPLC分析方法.以邻苯二甲醛(OPA)为衍生化试剂进行柱前衍生化,334nm作为检测波长,流动相为甲醇水溶液(V甲醇:V水=70:30),标准曲线为Y=1×10^6X+4.1238×10^4,r=0.9983,平均回收率为99.48%.用该方法测定发酵液中硫酸新霉素的含量取得了满意的结果.

Keyword:

柱前衍生化 硫酸新霉素 邻苯二甲醛(OPA)

Community:

  • [ 1 ] 福州大学生物科学与工程学院,福建福州350002

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Source :

福州大学学报:自然科学版

ISSN: 1000-2243

CN: 35-1337/N

Year: 2007

Issue: 1

Volume: 35

Page: 130-132

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 0

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